DeepNano: Deep Recurrent Neural Networks for Base Calling in MinION Nanopore Reads

The MinION device by Oxford Nanopore produces very long reads (reads over 100 kBp were reported); however it suffers from high sequencing error rate. We present an open-source DNA base caller based on deep recurrent neural networks and show that the accuracy of base calling is much dependent on the underlying software and can be improved by considering modern machine learning methods. By employing carefully crafted recurrent neural networks, our tool significantly improves base calling accuracy on data from R7.3 version of the platform compared to the default base caller supplied by the manufacturer. On R9 version, we achieve results comparable to Nanonet base caller provided by Oxford Nanopore. Availability of an open source tool with high base calling accuracy will be useful for development of new applications of the MinION device, including infectious disease detection and custom target enrichment during sequencing.

Tags
Data and Resources
To access the resources you must log in

This item has no data

Identity

Description: The Identity category includes attributes that support the identification of the resource.

Field Value
PID https://www.doi.org/10.1371/journal.pone.0178751
PID arXiv:1603.09195
PID pmc:PMC5459436
PID pmid:28582401
URL https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0178751&type=printable
URL https://128.84.21.199/abs/1603.09195
URL http://arxiv.org/abs/1603.09195
URL https://www.ncbi.nlm.nih.gov/pubmed/28582401
URL https://academic.microsoft.com/#/detail/2317495605
URL http://dx.plos.org/10.1371/journal.pone.0178751
URL https://arxiv.org/pdf/1603.09195.pdf
URL https://www.arxiv.org/abs/1603.09195
URL https://dx.plos.org/10.1371/journal.pone.0178751
URL http://europepmc.org/articles/PMC5459436?pdf=render
URL https://doaj.org/toc/1932-6203
URL http://dx.doi.org/10.1371/journal.pone.0178751
URL https://paperity.org/p/80223838/deepnano-deep-recurrent-neural-networks-for-base-calling-in-minion-nanopore-reads
URL http://europepmc.org/articles/PMC5459436
URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0178751
Access Modality

Description: The Access Modality category includes attributes that report the modality of exploitation of the resource.

Field Value
Access Right Open Access
Attribution

Description: Authorships and contributors

Field Value
Author Brona Brejova, 0000-0002-9483-1766
Author Tomas Vinar, 0000-0003-3898-3447
Contributor Zhi, Degui
Publishing

Description: Attributes about the publishing venue (e.g. journal) and deposit location (e.g. repository)

Field Value
Collected From PubMed Central; ORCID; UnpayWall; Datacite; arXiv.org e-Print Archive; DOAJ-Articles; Crossref; Microsoft Academic Graph
Hosted By Europe PubMed Central; PLoS ONE; arXiv.org e-Print Archive
Publication Date 2017-06-05
Additional Info
Field Value
Language English
Resource Type Other literature type; Preprint; Article
keyword Q
keyword R
keyword keywords.General Biochemistry, Genetics and Molecular Biology
system:type publication
Management Info
Field Value
Source https://science-innovation-policy.openaire.eu/search/publication?articleId=dedup_wf_001::ac38da5efb4d57b92cb374d5985ef6a0
Author jsonws_user
Last Updated 26 December 2020, 01:29 (CET)
Created 26 December 2020, 01:29 (CET)