(A–H) Representative image of BrdU incorporation into epithelial cells in the intestinal bulb of 124 hpf zebrafish embryos. BrdU staining in wild-type embryos (A) and embryos...

(A) Expression levels of MLLT10/AF10, β-catenin, DOT1L, and Tubulin analyzed by Western blotting after siRNA depletion of MLLT10/AF10. ChIP experiments in Ls174T CRCs containing...

(A) Schematic representation of the mouse Axin2 locus and amplicons scanned in Chromatin immunoprecipitation experiments by qPCR. Purified crypt and villus fractions from mouse...

Depletion of mllt10/af10 and dot1l rescues expression of i-fabp in apcmcr/mcr mutant embryos, placing these genes downstream of Apc as Wnt target gene activators (A–N)....

(A) Loss of MLLT10/AF10 abrogates TCF/LEF driven transcriptional activation. siRNA mediated depletion of MLLT10/AF10 reduces β-catenin/TCF driven transcription in Ls174T CRCs....

Cell lysates from Ls174T CRCs were immunoprecipitated with antibodies against endogenous MLLT10/AF10 (A) and DOT1L (B) complexes and analyzed by Western blotting with the...

(A–F) In situ hybridization for tcf7l2 (A), mllt10/af10 (C), and dot1l (E) in whole embryos at 80 hpf. In situ hybridization for tcf7l2 (B), mllt10/af10 (D), and dot1l (F) in...

(A) Cell lysates from purified crypt fractions were immunoprecipitated with antibodies directed against endogenous Tcf4, β-catenin, and (B) Mllt10/Af10 as indicated and analyzed...

Wnt-induced association of MLLT10/AF10-DOT1L with and regulation of Wnt target genes in HEK293T cells. (A–F) ChIP assays in HEK293T cells uninduced or induced with Wnt3A...