MLLT10/AF10 targets DOT1L-H3K79 methylation to Wnt target genes and is essential for transcription elongation.

(A) Expression levels of MLLT10/AF10, β-catenin, DOT1L, and Tubulin analyzed by Western blotting after siRNA depletion of MLLT10/AF10. ChIP experiments in Ls174T CRCs containing or depleted of MLLT10/AF10 by siRNA using antibodies against (B) TCF4, (C) β-catenin, (D) MLLT10/AF10, (E) DOT1L, (F) H3K79 dimethyl, (G) H3K79 trimethyl, (H) H3K4trimethyl, (I) RNA Pol II, (J) RNA Pol II Ser2P, and (K) RNA Pol II Ser5P. Immunoprecipitated DNA was analyzed by qPCR using primers specific for AXIN2 and c-MYC promoters and unbound AXIN2 upstream control region. Results are presented as percent immunoprecipitated over input and are representative of three independent experiments.

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PID https://www.doi.org/10.1371/journal.pbio.1000539.g006
URL https://figshare.com/articles/figure/_MLLT10_AF10_targets_DOT1L_H3K79_methylation_to_Wnt_target_genes_and_is_essential_for_transcription_elongation_/489202
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Author Mahmoudi, Tokameh
Author Boj, Sylvia F.
Author Hatzis, Pantelis
Author Li, Vivian S. W.
Author Taouatas, Nadia
Author Vries, Robert G. J.
Author Teunissen, Hans
Author Begthel, Harry
Author Korving, Jeroen
Author Mohammed, Shabaz
Author Heck, Albert J. R.
Author Clevers, Hans
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Collected From figshare
Hosted By figshare
Publication Date 2017-02-02
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Language English
Resource Type Image
keyword β- catenin-dependent manner
keyword β- catenin coactivators p 300
system:type dataset
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Source https://science-innovation-policy.openaire.eu/search/dataset?datasetId=r37980778c78::4dfeabc2fabecf58dbb18fca6cf3d702
Author jsonws_user
Last Updated 11 January 2021, 05:29 (CET)
Created 11 January 2021, 05:29 (CET)