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A Gateway MultiSite Recombination Cloning Toolkit
The generation of DNA constructs is often a rate-limiting step in conducting biological experiments. Recombination cloning of single DNA fragments using the Gateway system... -
False positive results of Bowie and Dick type test used for hospital steam st...
Objective To determine if the standardized B&D type test for hospital steam sterilizer is correctly indicating cycle failures in slower come-up ramps cycles. Methods... -
Identification of molecular determinants that govern distinct STIM2 activatio...
The endoplasmic reticulum (ER) Ca2+ sensors stromal interaction molecule 1 (STIM1) and STIM2, which connect ER Ca2+ depletion with extracellular Ca2+ influx, are crucial for the... -
Developing a set of strong intronic promoters for robust metabolic engineerin...
Background Red yeast species in the Rhodotorula/Rhodosporidium genus are outstanding producers of triacylglyceride and cell biomass. Metabolic engineering is expected to further... -
Control of Caenorhabditis elegans germ-line stem-cell cycling speed meets req...
Background Stem cells are thought to play a critical role in minimizing the accumulation of mutations, but it is not clear which strategies they follow to fulfill that... -
Harvesting prevascularized smooth muscle cell sheets from common polystyrene ...
Cell sheet engineering has recently emerged as a promising strategy for scaffold-free tissue engineering. However, the primary method of harvesting cell sheets using... -
A β-glucosidase hyper-production Trichoderma reesei mutant reveals a potentia...
Background The conversion of cellulose by cellulase to fermentable sugars for biomass-based products such as cellulosic biofuels, biobased fine chemicals and medicines is an... -
The SpikerBox: A Low Cost, Open-Source BioAmplifier for Increasing Public Par...
Although people are generally interested in how the brain functions, neuroscience education for the public is hampered by a lack of low cost and engaging teaching materials. To... -
Using a vector pool containing variable-strength promoters to optimize protei...
Background The yeast Yarrowia lipolytica is an increasingly common biofactory. To enhance protein expression, several promoters have been developed, including the constitutive... -
Unilateral versus bilateral pedicle screw fixation in lumbar fusion: A system...
Objectives To carry out a systematic review on the basis of overlapping meta-analyses that compare unilateral with bilateral pedicle screw fixation (PSF) in lumbar fusion to... -
Traction force microscopy of engineered cardiac tissues
Cardiac tissue development and pathology have been shown to depend sensitively on microenvironmental mechanical factors, such as extracellular matrix stiffness, in both in vivo... -
NanoR: a user-friendly R package to analyze and compare nanopore sequencing data
AbstractMinION and GridION X5 from Oxford Nanopore Technologies are devices for real-time DNA and RNA sequencing. On the one hand, MinION is the only real-time, low cost and... -
Impact of degradable nanowires on long-term brain tissue responses
Background A promising approach to improve the performance of neural implants consists of adding nanomaterials, such as nanowires, to the surface of the implant. Nanostructured... -
Foldscope: Origami-Based Paper Microscope
Here we describe an ultra-low-cost origami-based approach for large-scale manufacturing of microscopes, specifically demonstrating brightfield, darkfield, and fluorescence... -
Precise cloning and tandem integration of large polyketide biosynthetic gene ...
Background Direct cloning combined with heterologous expression of a secondary metabolite biosynthetic gene cluster has become a useful strategy for production improvement and... -
Trial registration, publication rate and characteristics in the research fiel...
OBJECTIVES: To examine 1) the publication rate of registered otology trials in ClinicalTrials.gov, 2) the public availability of the results, 3) the study characteristics... -
Use of three points to determine the accuracy of guided implantation
AIM:'This study aims to establish an open-source algorithm using Python to analyze the accuracy of guided implantation, which simplifies interstudy comparisons. METHODS:Given ≥3... -
De novo production of six key grape aroma monoterpenes by a geraniol synthase...
[Background] Monoterpenes are important contributors to grape and wine aroma. Moreover, certain monoterpenes have been shown to display health benefits with antimicrobial,... -
Comparison and evaluation of the morphology of crowns generated by biogeneric...
OBJECTIVES: To better guide clinicians to choose the appropriate chairside system, we compared and evaluated the morphology of crowns generated by three different biogeneric... -
Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant pr...
Background: In recent years, the non-conventional model yeast species Yarrowia lipolytica has received much attention because it is a useful cell factory for producing...
