De novo production of six key grape aroma monoterpenes by a geraniol synthase-engineered S. cerevisiae wine strain.

[Background] Monoterpenes are important contributors to grape and wine aroma. Moreover, certain monoterpenes have been shown to display health benefits with antimicrobial, anti-inflammatory, anticancer or hypotensive properties amongst others. The aim of this study was to construct self-aromatizing wine yeasts to overproduce de novo these plant metabolites in wines.

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PID pmid:26377186
PID https://www.doi.org/10.1186/s12934-015-0306-5
PID pmc:PMC4574175
PID handle:10261/125683
URL https://digital.csic.es/bitstream/10261/125683/1/Pardo-Microb%20Cell%20Fact-2015.pdf
URL https://microbialcellfactories.biomedcentral.com/track/pdf/10.1186/s12934-015-0306-5
URL https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-015-0306-5
URL https://academic.microsoft.com/#/detail/2130601565
URL http://hdl.handle.net/10261/125683
URL https://link.springer.com/content/pdf/10.1186%2Fs12934-015-0306-5.pdf
URL https://www.ncbi.nlm.nih.gov/pubmed/26377186
URL https://link.springer.com/article/10.1186/s12934-015-0306-5
URL http://europepmc.org/articles/PMC4574175
URL http://www.microbialcellfactories.com/content/14/1/136
URL https://paperity.org/p/73953066/de-novo-production-of-six-key-grape-aroma-monoterpenes-by-a-geraniol-synthase-engineered
URL https://dx.doi.org/10.1186/s12934-015-0306-5
URL http://digital.csic.es/bitstream/10261/125683/1/Pardo-Microb%20Cell%20Fact-2015.pdf
URL http://digital.csic.es/handle/10261/125683
URL http://link.springer.com/content/pdf/10.1186/s12934-015-0306-5
URL http://dx.doi.org/10.1186/s12934-015-0306-5
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Access Right Open Access
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Author José Vicente Gil, 0000-0003-2062-3435
Author Juan Rico, 0000-0003-0317-1043
Contributor Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Contributor European Commission
Contributor Ministerio de Economía y Competitividad (España)
Contributor CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
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Collected From Europe PubMed Central; PubMed Central; Digital.CSIC; ORCID; Datacite; UnpayWall; Crossref; Microsoft Academic Graph; CORE (RIOXX-UK Aggregator)
Hosted By Europe PubMed Central; SpringerOpen; Digital.CSIC; Microbial Cell Factories
Publication Date 2015-09-16
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Country Spain
Description [Results] Expression of the Ocimum basilicum (sweet basil) geraniol synthase (GES) gene in a Saccharomyces cerevisiae wine strain substantially changed the terpene profile of wine produced from a non-aromatic grape variety. Under microvinification conditions, and without compromising other fermentative traits, the recombinant yeast excreted geraniol de novo at an amount (~750 μg/L) well exceeding (>10-fold) its threshold for olfactory perception and also exceeding the quantities present in wines obtained from highly aromatic Muscat grapes. Interestingly, geraniol was further metabolized by yeast enzymes to additional monoterpenes and esters: citronellol, linalool, nerol, citronellyl acetate and geranyl acetate, resulting in a total monoterpene concentration (~1,558 μg/L) 230-fold greater than that of the control. We also found that monoterpene profiles of wines derived from mixed fermentations were found to be determined by the composition of the initial yeast inocula suggesting the feasibility of producing ‘à la carte’ wines having predetermined monoterpene contents.
Description [Conclusions] Geraniol synthase-engineered yeasts demonstrate potential in the development of monoterpene enhanced wines.
Description This work was funded by the Spanish MINECO/FEDER Grants CSD2007-0063 and AGL2011-29925. We also acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). E. Pardo was financially supported by JCI2007-123/733 and JAEDOC086-FSE contracts. J. Rico was the recipient of a FPI (Formación de Personal de Investigador) pre-doctoral fellowship from the Comisión Interministerial de Ciencia y Tecnología (CICYT).
Description Peer Reviewed
Language Undetermined
Resource Type Article; UNKNOWN
keyword mesheuropmc.digestive, oral, and skin physiology
system:type publication
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Source https://science-innovation-policy.openaire.eu/search/publication?articleId=dedup_wf_001::88c8edc4bf9005effa00f268ede583e8
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Last Updated 23 December 2020, 11:17 (CET)
Created 23 December 2020, 11:17 (CET)