r37980778c78--7cde987632e3675a6ed618a7d6d1b5de

(A) The previously published pPax8-rtTA plasmid [7] is shown in the top panel. The CreERT2 coding sequence followed by a polyadenylation signal (pA) was used to replace the rtTA sequence of pPax8-rtTA and generate plasmid pPax8-CreERT2 (bottom panel) containing 4.3kb of upstream regulatory sequence, complete exon 1 and intron 1, part of exon 2 and 0.8kb of intron 2 of the murine Pax8 gene. (B) FISH analysis of metaphase spreads using pPax8-CreERT2 as probe (green) maps the integration site to chromosome 6 (arrowheads). A weaker signal from the endogenous Pax8 locus can be detected on chromosome 2 (arrows).

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PID https://www.doi.org/10.1371/journal.pone.0148055.g001
URL http://dx.doi.org/10.1371/journal.pone.0148055.g001
URL https://figshare.com/articles/_Generation_of_Pax8_CreER_T2_transgenic_mice_/1644760
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Collected From figshare
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Publication Date 2016-03-16
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Source https://science-innovation-policy.openaire.eu/search/dataset?datasetId=r37980778c78::7cde987632e3675a6ed618a7d6d1b5de
Author jsonws_user
Last Updated 10 January 2021, 20:58 (CET)
Created 10 January 2021, 20:58 (CET)