Activated caspase-3 immunohistochemistry shows that Thy1-p45 mice have reduced cell death following stab wound injury compared to their WT littermates.

One (A, B) or 3 days (C, D) after stab wound injuries on the right side of T13 spinal cord, Thy1-p45 mice (B, D) had fewer activated caspase-3+ cells compared to their WT littermates (A, C) on the injured side of the spinal cord. Serving as an internal control, there were almost no activated caspase-3+ cells on the contralateral uninjured side of the spinal cord in every section. The activated caspase-3+ cells co-localized with Hoechest 33342 nuclei staining and showed the typical features of cell death (DNA condensation) in the magnified view of injured spinal cord from both Thy1-p45 mice and their WT littermates (E, F). Scale bars: 200 µm (A–D), 20 µm (E, F).

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PID https://www.doi.org/10.1371/journal.pone.0069286.g005
URL http://dx.doi.org/10.1371/journal.pone.0069286.g005
URL https://figshare.com/articles/_Activated_caspase_3_immunohistochemistry_shows_that_Thy1_p45_mice_have_reduced_cell_death_following_stab_wound_injury_compared_to_their_WT_littermates_/752959
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Author Sung, Tsung-Chang
Author Chen, Zhijiang
Author Thuret, Sandrine
Author Vilar, Marçal
Author H. Gage, Fred
Author Riek, Roland
Author Lee, Kuo-Fen
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Publication Date 2016-01-01
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Source https://science-innovation-policy.openaire.eu/search/dataset?datasetId=r37980778c78::65127452d9a03aa131f98303ec48d6f1
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Last Updated 14 January 2021, 14:42 (CET)
Created 14 January 2021, 14:42 (CET)