Reduction of spherical-aberration impact in microscopy by wavefront coding

In modern high-NA optical scanning instruments, like scanning microscopes, the refractive-index mismatch between the sample and the immersion medium introduces a significant amount of spherical aberration when imaging deep inside the specimen, spreading out the impulse response. Since such aberration depends on the focalization depth, it is not possible to achieve a static global compensation for the whole 3D sample in scanning microscopy. Therefore a depth-variant impulse response is generated. Consequently, the design of pupil elements that increase the tolerance to this aberration is of great interest. In this paper we report a hybrid technique that provides a focal spot that remains almost invariant in the depth-scanning processing of thick samples. This invariance allows the application of 3D deconvolution techniques to that provide an improved recovery of the specimen structure when imaging thick samples.

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PID https://www.doi.org/10.6084/m9.figshare.c.3761879
PID https://www.doi.org/10.6084/m9.figshare.c.3761879.v1
URL http://dx.doi.org/10.6084/m9.figshare.c.3761879
URL http://dx.doi.org/10.6084/m9.figshare.c.3761879.v1
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Author G. Saavedra
Author I. Escobar
Author R. Martínez-Cuenca
Author E. Sánchez-Ortiga
Author M. Martínez-Corral
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Collected From Datacite
Hosted By figshare
Publication Date 2009-01-01
Publisher Figshare
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keyword FOS: Biological sciences
keyword FOS: Health sciences
keyword FOS: Physical sciences
system:type other
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Source https://science-innovation-policy.openaire.eu/search/other?orpId=dedup_wf_001::f6c32603b3a545e3f65eb3231cb0b1ba
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Last Updated 20 December 2020, 03:17 (CET)
Created 20 December 2020, 03:17 (CET)