Additional file 8. Representative images of genuine S-cones, genuine M/L-cones and dual cones in the superior peripheral retina of Rd1 mice at postnatal day 14. Double labeling immunofluorescence of retinal wholemounts was performed using antibodies directed against S-opsin (red) and M/L-opsin (green). (A) S-opsin+ cones; (B) M/L-opsin+ cones; (C) merged image (all cones); (D) mask of genuine S-cones, (E) mask of genuine M/L-cones (F) mask of dual cones; (G) merged image (all cones) overlaid with mask of genuine S-cones; (H) merged image (all cones) overlaid with mask of genuine M/L-cones; (I) merged image (all cones) overlaid with mask of dual cones. Scale bar: 100 Îźm.