Data from legal records, management plans, and interviews with 63 local experts reveal the substantial expansion of 15 protected areas (PAs) of forest in Ecuador and Peru during the last two decades. Combining results for these PAs, the area under protection increased by over half, from 5,760,814 to 8,972,896 ha, with the Amazonian PAs adding the greatest expanse. Most of this expanded land was legally designated for strict protection; however, in practice, human resource use and settlement are widespread. Hunting is the most common resource use, followed by logging and livestock grazing. Mining and petroleum extraction also occur in four of the 15 PAs. Together these activities on average affect approximately 30% of the area within eight Peruvian PAs and approximately 45% of the area of seven Ecuadorian PAs, far exceeding previous deforestation estimates. By expanding these PAs, Ecuadorian and Peruvian conservationists have significantly improved the coverage of key ecosystems and endangered habitats. However, they now face the daunting task of managing larger, more complex protected areas that de facto include thousands of local people. Conservation agencies in both countries are turning toward land-use zoning within PAs to integrate resource use with biodiversity conservation.