Gating strategy for identifying dopaminergic and adrenergic receptors on T cells. Spleens were isolated from mice 15 d.p.i and analyzed by flow cytometry for dopamine and adrenergic receptor expression. A gating strategy for identifying CD4 (bottom left), CD8 (bottom center), and Tregs (bottom right). Representative histograms for expression of B dopamine D1 receptor (D1R) and C dopamine D2 receptor (D2R) on CD4 T cells. Graphical representation of receptor expression on different T cell subsets of D D1R and E D2R. Shown are means and SEM of individual mice from one experiment (n = 5 mice in each group). Statistical analyses between healthy control and EAE mice conducted by two-way ANOVA. (TIF 17402 kb)