Figure S1. Temporal stability of fecal microbiota and cage effect of the immunizations. Weighted Unifrac distances of mice fecal microbial communities represented over the time course of the experiment according to the immunization (A) or the cage (B). Permutational MANOVA of weighted Unifrac distances according to timepoint, immunizations, caging, and their interactions (time × cage; time × immunization; immunization × cage) as described in Additional file 2. The time had a significant effect on the stability of the fecal microbiota (P = 0.001), in particular between the baseline (T0) and the last timepoint (T4, P = 0.003); and between the T1 and T4 (P = 0.009). The interaction between time and immunization was significant (P = 0.007). Cage was also significant, in particular the interaction cage × timepoint (P = 0.001) and cage × immunization (P = 0.002). Significant differences within the same immunization group cage has been observed (TSHR group in C4 and C5, P = 0.01). (PDF 152 kb)