Additional file 5: Figure S4. Lowering the dose of Notch partially rescues lgl-IR-induced MMP1 expression and restores the adult wing. a MMP1 expression in wild-type wing disc is shown. b Overexpression of only Notch-DN did not induce expression of MMP1. c Overexpression of lgl-IR induces MMP1 expression in the wing disc. d Coexpression of Notch-DN in lgl-IR background partially rescues the expression of MMP1 caused by lgl-IR overexpression. a′, b′, c′ and d′ are merges of DAPI along with a, b, c and d, respectively. Moreover, Coexpression of Notch-DN with lgl-IR resulted in reduced wing disc size as compared to that of only overexpression of lgl-IR (i). e GFP marked vestigial domain in wing disc is shown. e′ is the merge image of DAPI along with (e). f Overexpression of Notch-DN resulted in held out wings with wing nicking phenotype. g Overexpression of lgl-IR using vg-GAL4 led to necrotic lesions followed by deformation of adult wings. h Coexpression of Notch-DN with lgl-IR partially restored deformed adult wings. j Phenotype penetrance in adult flies is shown for each genotype; the phenotype observed in Notch-DN show 100% penetrance and around 70% lgl-IR flies showed deformed wings. In case of Notch-DN; lgl-IR flies, around 60% flies showed the depicted phenotype and, the rest of the flies showed less developed wings but they were not of the lgl-IR category. Analysis of data was done using One-way ANOVA with Tukey’s multiple comparison test; data represents mean ± SEM (***p  0.05). All wing discs are oriented with dorsal to the top and posterior to the right. Scale bar: 50 µm (a–d, a′–d′).