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Development of a promoter shutoff system in <i>Aspergillus oryzae</i> using a...
Promoter shutoff is a general method for analyzing essential genes, but in the fungus Aspergillus oryzae, no tightly repressed promoters have been reported. To overcome the... -
s·nr: a visual analytics framework for contextual analyses of private and pub...
Abstract Background Next-Generation Sequencing (NGS) has been widely accepted as an essential tool in molecular biology. Reduced costs and automated analysis pipelines make the... -
Condition-specific promoter activities in Saccharomyces cerevisiae
Abstract Background Saccharomyces cerevisiae is widely studied for production of biofuels and biochemicals. To improve production efficiency under industrially relevant... -
Golgi tethering factor golgin-97 suppresses breast cancer cell invasiveness b...
Abstract Background Golgin-97 is a tethering factor in the trans-Golgi network (TGN) and is crucial for vesicular trafficking and maintaining cell polarity. However, the... -
Genetic tool for fate mapping of Oct4 (Pou5f1)-expressing cells and their pro...
Abstract Background Methods based on site-specific recombinases are widely used in studying gene activities in vivo and in vitro. In these studies, constitutively active or... -
Role and expression of <i>cry1</i> in the adductor muscle of the oyster <i>Cr...
Cryptochromes are flavin- and pterin-containing photoreceptors of the cryptochrome/photolyase family. They play critical roles in organisms, among are which light-dependent and... -
Omalizumab in patients with severe asthma and persistent sputum eosinophilia
Abstract Omalizumab, a recombinant humanized monoclonal antibody targeting the IgE molecule, is the first biologic approved for moderate-to-severe allergic asthmatics, who... -
Genome-wide DNA methylation at birth in relation to in utero arsenic exposure...
Abstract Background In utero arsenic exposure may alter fetal developmental programming by altering DNA methylation, which may result in a higher risk of disease in later life.... -
Molecular characteristics of Staphylococcus aureus isolates from food surveil...
Abstract Background Staphylococcal food poisoning (SFP) is one of the most common food-borne diseases in the world. Pulsed-field gel electrophoresis (PFGE), multilocus sequence... -
Novel mutation in the CHST6 gene causes macular corneal dystrophy in a black ...
Abstract Background Macular corneal dystrophy (MCD) is a rare autosomal recessive disorder that is characterized by progressive corneal opacity that starts in early childhood... -
Comparative transcriptomic analyses of normal and malformed flowers in sugar ...
Abstract Background Sugar apple (Annona squamosa L.), a popular fruit with high medicinal and nutritional properties, is widely cultivated in tropical South Asia and America.... -
Boron-deficiency-responsive microRNAs and their targets in Citrus sinensis le...
Abstract Background MicroRNAs play important roles in the adaptive responses of plants to nutrient deficiencies. Most research, however, has focused on nitrogen (N), phosphorus... -
Managing or restricting movement? Diverging approaches of African and Europea...
Abstract Though a common EU-African agenda of dealing with migration goes back at least a decade with bilateral, regional and continental approaches, it has gained in visible... -
Effects of the Toll-like receptor 7 (TLR7) agonist, AZD8848, on allergen-indu...
Abstract Background Although allergic asthma is a complex area with many interacting factors involved, the ‘hygiene hypothesis’ proposes that a lack of exposure to infection... -
Patternable particle microarray utilizing controllable particle delivery
Abstract In this study, we demonstrate an on-demand delivering and sequential arraying of single microparticles utilizing multiple pneumatic pressure-driven elastomer valves and... -
Excessive carbohydrate consumption and body mass index: the risk factors for ...
Abstract Background Prader-Willi syndrome (PWS) is commonly associated with severe obesity and type 2 diabetes mellitus (T2DM) and is caused by excessive eating. Only very few... -
Retrospective analysis in oculocutaneous albinism patients for the 2.7 kb del...
Abstract Background Oculocutaneous albinism (OCA) is an autosomal recessive disorder. A significant portion of OCA patients has been found with a single pathogenic variant... -
Bone marrow failure may be caused by chromosome anomalies exerting effects on...
Abstract Background The majority of the cases of bone marrow failure syndromes/aplastic anaemias (BMFS/AA) are non-hereditary and considered idiopathic (80â 85%). The... -
CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cl...
Abstract Background The CRISPR/dCas9 system is a powerful tool to activate the transcription of target genes in eukaryotic or prokaryotic cells, but lacks assays in complex... -
Efficient production of extracellular pullulanase in Bacillus subtilis ATCC60...
Abstract Background Bacillus subtilis has been widely used as a host for heterologous protein expression in food industry. B. subtilis ATCC6051 is an alternative expression host...
