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Quality filtering of Illumina index reads mitigates sample cross-talk
Background Multiplexing multiple samples during Illumina sequencing is a common practice and is rapidly growing in importance as the throughput of the platform increases.... -
Unique, dual-indexed sequencing adapters with UMIs effectively eliminate inde...
Background: Sample index cross-talk can result in false positive calls when massively parallel sequencing (MPS) is used for sensitive applications such as low-frequency somatic...